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ATAD1 Recombinant antibody

ATAD1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241449D12

Cat no : 84194-6-RR

Print datasheet

Synonyms

FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1

验证数据展示

  • WB analysis using 84194-6-RR

    WB analysis using 84194-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84194-6-RR (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84194-6-RR

    IF Staining of HeLa using 84194-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATAD1 antibody (84194-6-RR, Clone: 241449D12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 84194-6-RR

    FC experiment of A431 using 84194-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ATAD1 Recombinant antibody (84194-6-RR, Clone:241449D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84194-6-RR

    Affinity and Kinetic Characterization of 84194-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84194-6-RR against Human ATAD1 were performed. The affinity constant is 0.573 nM.

经过测试的应用

Positive WB detected inLNCaP cells, A549 cells, Jurkat cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84194-6-RR is ATAD1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATAD1 fusion protein Ag10343 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase family, AAA domain containing 1
Synonyms FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1
Calculated Molecular Weight 361 aa, 41 kDa
Observed Molecular Weight32-37 kDa
GenBank Accession NumberBC010868
Gene Symbol ATAD1
Gene ID (NCBI) 84896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NBU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.

实验方案

Product Specific Protocols
WB protocol for ATAD1 antibody 84194-6-RRDownload protocol
IF protocol for ATAD1 antibody 84194-6-RRDownload protocol
FC protocol for ATAD1 antibody 84194-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols