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CoraLite®594-conjugated ATF6 Monoclonal antibody
ATF6 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3B7E4
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ATF6 Ready-To-Use IHC Kit. ATF6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66563-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66563 targets ATF6 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | ATF6 fusion protein Ag21456 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | activating transcription factor 6 |
Synonyms | ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6 |
Calculated Molecular Weight | 75 kDa |
Observed Molecular Weight | 90-100 kDa |
GenBank Accession Number | BC014969 |
Gene Symbol | ATF6 |
Gene ID (NCBI) | 22926 |
RRID | AB_2920006 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P18850 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 ATF6 antibody CL594-66563 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |