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CoraLite® Plus 488-conjugated ATF6 Monoclonal antibody

ATF6 Monoclonal Antibody for IF/ICC
Cat No. CL488-66563

产品说明书

CloneNo. 3B7E4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ATF6 Ready-To-Use IHC Kit. ATF6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66563-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66563 targets ATF6 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 ATF6 fusion protein Ag21456 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 activating transcription factor 6
别名 ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6
计算分子量 75 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC014969
基因名称 ATF6
Gene ID (NCBI) 22926
RRIDAB_2919344
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP18850
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ATF6 antibody CL488-66563Download protocol
Standard Protocols
Click here to view our Standard Protocols
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