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验证数据展示

  • FC experiment of HeLa using CL488-66708

    FC experiment of HeLa using CL488-66708

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ATG13 (CL488-66708, Clone:1C3A7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-66708

    IF Staining of HeLa using CL488-66708

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ATG13 antibody (CL488-66708, Clone: 1C3A7 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ATG13 Monoclonal antibody

ATG13 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66708

产品说明书

CloneNo. 1C3A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

KIAA0652, 1C3A7, Autophagy related protein 13, Autophagy-related protein 13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66708-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66708 targets ATG13 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ATG13 fusion protein Ag12968 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA0652
别名 KIAA0652, 1C3A7, Autophagy related protein 13, Autophagy-related protein 13
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC001331
基因名称 ATG13
Gene ID (NCBI) 9776
RRIDAB_2883373
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75143
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ATG13 antibody CL488-66708Download protocol
FC protocol for CL Plus 488 ATG13 antibody CL488-66708Download protocol
Standard Protocols
Click here to view our Standard Protocols