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CoraLite®594-conjugated ATG13 Monoclonal antibody

ATG13 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C3A7

Cat no : CL594-66708

Print datasheet

Synonyms

ATG13, Autophagy related protein 13, FLJ20698, KIAA0652

验证数据展示

  • IF Staining of HeLa using CL594-66708

    IF Staining of HeLa using CL594-66708

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 ATG13 antibody (CL594-66708, Clone: 1C3A7 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66708-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66708 targets ATG13 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ATG13 fusion protein Ag12968 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name KIAA0652
Synonyms ATG13, Autophagy related protein 13, FLJ20698, KIAA0652
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC001331
Gene Symbol ATG13
Gene ID (NCBI) 9776
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75143
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

实验方案

Product Specific Protocols
IF protocol for CL594 ATG13 antibody CL594-66708Download protocol
Standard Protocols
Click here to view our Standard Protocols