验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66708-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66708 targets ATG13 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat, Pig |
| 免疫原 | ATG13 fusion protein Ag12968 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0652 |
| 别名 | ATG13, Autophagy related protein 13, FLJ20698, KIAA0652 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC001331 |
| 基因名称 | ATG13 |
| Gene ID (NCBI) | 9776 |
| RRID | AB_2923924 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O75143 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 ATG13 antibody CL594-66708 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |