验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy ATP1B3 Ready-To-Use IHC Kit. ATP1B3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67554-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67554 targets ATP1B3 in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | ATP1B3 fusion protein Ag30127 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, Na+/K+ transporting, beta 3 polypeptide |
| 别名 | ATP1B3, ATPB 3, CD298, FLJ29027 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 38-43 kDa |
| GenBank蛋白编号 | BC011835 |
| 基因名称 | ATP1B3 |
| Gene ID (NCBI) | 483 |
| RRID | AB_3084355 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P54709 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATP1B3 antibody CL488-67554 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |