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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ATP1B3 Monoclonal antibody

ATP1B3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H9C1

Cat no : CL488-67554

Print datasheet

Synonyms

ATP1B3, ATPB 3, CD298, FLJ29027

验证数据展示

  • IF Staining of HEK-293 using CL488-67554

    IF Staining of HEK-293 using CL488-67554

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ATP1B3 antibody (CL488-67554, Clone: 1H9C1 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ATP1B3 Ready-To-Use IHC Kit. ATP1B3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67554-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67554 targets ATP1B3 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen ATP1B3 fusion protein Ag30127 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATPase, Na+/K+ transporting, beta 3 polypeptide
Synonyms ATP1B3, ATPB 3, CD298, FLJ29027
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberBC011835
Gene Symbol ATP1B3
Gene ID (NCBI) 483
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP54709
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ATP1B3 antibody CL488-67554Download protocol
Standard Protocols
Click here to view our Standard Protocols