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  • Featured Product
  • KD/KO Validated

ATP5A1 Monoclonal antibody, PBS Only

ATP5A1 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, monkey

Applications

WB,IP,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

1B10H3

Cat No : 66037-1-PBS

Print datasheet

Synonyms

ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Maarker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM

验证数据展示

  • IF Staining of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

    IF Staining of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:300 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: CL555-phalloidin staining of F-actin. . This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • WB analysis using 66037-1-Ig (same clone as 66037-1-PBS)

    WB analysis using 66037-1-Ig (same clone as 66037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

    IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP5A1 antibody included
  • IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

    IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP5A1 antibody included
  • IP experiment of mouse heart using 66037-1-Ig (same clone as 66037-1-PBS)

    IP experiment of mouse heart using 66037-1-Ig (same clone as 66037-1-PBS)

    IP result of anti-ATP5A1 (IP:66037-1-Ig, 5ug; Detection:66037-1-Ig 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66037-1-Ig (same clone as 66037-1-PBS)

    FC experiment of HeLa using 66037-1-Ig (same clone as 66037-1-PBS)

    1X10^6 HeLa cells were stained with 0.2 ug Anti-Human ATP5A1 (66037-1-Ig, Clone:1B10H3) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

    WB analysis of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

    WB result of ATP5A1 antibody (66037-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP5A1 transfected HepG2 cells. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66037-1-Ig (same clone as 66037-1-PBS)

    IHC staining of human liver cancer using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP5A1 antibody included
Planning an IHC experiment? We recommend our IHCeasy ATP5A1 Ready-To-Use IHC Kit. ATP5A1 primary antibody included.

产品信息

66037-1-PBS targets ATP5A1 in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF,FC
Tested Reactivity human, mouse, rat, monkey
Immunogen ATP5A1 fusion protein Ag8119 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Synonyms ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Maarker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC064562
Gene Symbol ATP5A1
Gene ID (NCBI) 498
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25705
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.