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验证数据展示

  • IF Staining of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

    IF Staining of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:300 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: CL555-phalloidin staining of F-actin. . This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • WB analysis using 66037-1-Ig (same clone as 66037-1-PBS)

    WB analysis using 66037-1-Ig (same clone as 66037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

    IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP5A1 antibody included
  • IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

    IHC staining of human heart using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP5A1 antibody included
  • IP experiment of mouse heart using 66037-1-Ig (same clone as 66037-1-PBS)

    IP experiment of mouse heart using 66037-1-Ig (same clone as 66037-1-PBS)

    IP result of anti-ATP5A1 (IP:66037-1-Ig, 5ug; Detection:66037-1-Ig 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

    WB analysis of HepG2 using 66037-1-Ig (same clone as 66037-1-PBS)

    WB result of ATP5A1 antibody (66037-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP5A1 transfected HepG2 cells. This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66037-1-Ig (same clone as 66037-1-PBS)

    IHC staining of human liver cancer using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP5A1 antibody included
  • IF Staining of HeLa using 66037-1-Ig (same clone as 66037-1-PBS)

    IF Staining of HeLa using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (66037-1-Ig, Clone: 1B10H3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66037-1-Ig (same clone as 66037-1-PBS)

    IF Staining of HeLa using 66037-1-Ig (same clone as 66037-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (66037-1-Ig, Clone: 1B10H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66037-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ATP5A1 Monoclonal antibody, PBS Only

ATP5A1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66037-1-PBS

产品说明书

CloneNo. 1B10H3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

ATP5A 1, ATP5A, ATP synthase F1 subunit alpha, 1B10H3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ATP5A1 Ready-To-Use IHC Kit. ATP5A1 primary antibody included.

产品信息

66037-1-PBS targets ATP5A1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原 ATP5A1 fusion protein Ag8119 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
别名 ATP5A 1, ATP5A, ATP synthase F1 subunit alpha, 1B10H3
计算分子量 60 kDa
观测分子量 50 kDa
GenBank蛋白编号BC064562
基因名称 ATP5A1
Gene ID (NCBI) 498
RRIDAB_11044196
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25705
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.