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ATP5A1 Monoclonal antibody
ATP5A1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, monkey and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B10H3
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human liver cancer tissue, human liver tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66037-1-Ig targets ATP5A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat, monkey |
Cited Reactivity | human, mouse, rat, hamster |
Immunogen | ATP5A1 fusion protein Ag8119 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle |
Synonyms | ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Maarker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM |
Calculated Molecular Weight | 60 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC064562 |
Gene Symbol | ATP5A1 |
Gene ID (NCBI) | 498 |
RRID | AB_11044196 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P25705 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ATP5A1 antibody 66037-1-Ig | Download protocol |
IHC protocol for ATP5A1 antibody 66037-1-Ig | Download protocol |
IF protocol for ATP5A1 antibody 66037-1-Ig | Download protocol |
IP protocol for ATP5A1 antibody 66037-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1. | ||
Nat Commun The non-redundant functions of PIWI family proteins in gametogenesis in golden hamsters | ||
Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine. | ||
Autophagy AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells. | ||
Elife Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells. |