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CoraLite®594-conjugated ATP5A1 Monoclonal antibody

ATP5A1 Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B10H3

Cat no : CL594-66037

Print datasheet

Synonyms

ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ATP5A1 Ready-To-Use IHC Kit. ATP5A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66037-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66037 targets ATP5A1 in WB, IF/ICC applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IF/ICC Application Description
Tested Reactivity human, mouse, rat, monkey
Immunogen ATP5A1 fusion protein Ag8119 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Synonyms ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC064562
Gene Symbol ATP5A1
Gene ID (NCBI) 498
RRIDAB_2919941
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25705
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CL594 ATP5A1 antibody CL594-66037Download protocol
IF protocol for CL594 ATP5A1 antibody CL594-66037Download protocol
Standard Protocols
Click here to view our Standard Protocols