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CoraLite® Plus 488-conjugated ATP5A1 Monoclonal antibody
ATP5A1 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, monkey
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B10H3
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66037 targets ATP5A1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat, monkey |
Immunogen | ATP5A1 fusion protein Ag8119 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle |
Synonyms | ATP5A 1, ATP5A, ATP synthase F1 subunit alpha, 1B10H3 |
Calculated Molecular Weight | 60 kDa |
GenBank Accession Number | BC064562 |
Gene Symbol | ATP5A1 |
Gene ID (NCBI) | 498 |
RRID | AB_2883234 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P25705 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 ATP5A1 antibody CL488-66037 | Download protocol |
FC protocol for CL Plus 488 ATP5A1 antibody CL488-66037 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |