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ATP6V1B2 Monoclonal antibody

ATP6V1B2 Monoclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

3C11A9

Cat no : 68441-1-Ig

Print datasheet

Synonyms

V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2

验证数据展示

  • WB analysis using 68441-1-Ig

    WB analysis using 68441-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68441-1-Ig (ATP6V1B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of A549 using 68441-1-Ig

    WB analysis of A549 using 68441-1-Ig

    WB result of ATP6V1B2 antibody (68441-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected A549 cells.

  • IP experiment of HeLa using 68441-1-Ig

    IP experiment of HeLa using 68441-1-Ig

    IP result of anti-ATP6V1B2 (IP:68441-1-Ig, 4ug; Detection:68441-1-Ig 1:4000) with HeLa cells lysate 2000 ug.

  • FC experiment of HeLa using 68441-1-Ig

    FC experiment of HeLa using 68441-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug ATP6V1B2 Monoclonal antibody (68441-1-Ig, Clone:3C11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, mouse brain tissue
Positive IP detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68441-1-Ig targets ATP6V1B2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen ATP6V1B2 fusion protein Ag7265 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Synonyms V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56-58 kDa
GenBank Accession NumberBC003100
Gene Symbol ATP6V1B2
Gene ID (NCBI) 526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21281
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).

实验方案

Product Specific Protocols
WB protocol for ATP6V1B2 antibody 68441-1-IgDownload protocol
IP protocol for ATP6V1B2 antibody 68441-1-IgDownload protocol
FC protocol for ATP6V1B2 antibody 68441-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols