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  • Featured Product
  • KD/KO Validated

ATP6V1B2 Monoclonal antibody, PBS Only

ATP6V1B2 Monoclonal Antibody for Indirect ELISA, IP, WB

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

Indirect ELISA, IP, WB

Conjugate

Unconjugated

CloneNo.

3C11A9

Cat no : 68441-1-PBS

Print datasheet

Synonyms

V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2

验证数据展示

  • WB analysis using 68441-1-Ig (same clone as 68441-1-PBS)

    WB analysis using 68441-1-Ig (same clone as 68441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68441-1-Ig (ATP6V1B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 68441-1-Ig (same clone as 68441-1-PBS)

    WB analysis of A549 using 68441-1-Ig (same clone as 68441-1-PBS)

    WB result of ATP6V1B2 antibody (68441-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected A549 cells. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

    IP experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

    IP result of anti-ATP6V1B2 (IP:68441-1-Ig, 4ug; Detection:68441-1-Ig 1:4000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

    FC experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug ATP6V1B2 Monoclonal antibody (68441-1-Ig, Clone:3C11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

产品信息

68441-1-PBS targets ATP6V1B2 in Indirect ELISA, IP, WB applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Applications Indirect ELISA, IP, WB Application Description
Tested Reactivity rabbit, pig, rat, mouse, human
Immunogen ATP6V1B2 fusion protein Ag7265 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Synonyms V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56-58 kDa
GenBank Accession NumberBC003100
Gene Symbol ATP6V1B2
Gene ID (NCBI) 526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21281
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).