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验证数据展示

  • WB analysis using 68441-1-Ig (same clone as 68441-1-PBS)

    WB analysis using 68441-1-Ig (same clone as 68441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68441-1-Ig (ATP6V1B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 68441-1-Ig (same clone as 68441-1-PBS)

    WB analysis of A549 using 68441-1-Ig (same clone as 68441-1-PBS)

    WB result of ATP6V1B2 antibody (68441-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected A549 cells. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

    IP experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

    IP result of anti-ATP6V1B2 (IP:68441-1-Ig, 4ug; Detection:68441-1-Ig 1:4000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

    FC experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug ATP6V1B2 Monoclonal antibody (68441-1-Ig, Clone:3C11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ATP6V1B2 Monoclonal antibody, PBS Only

ATP6V1B2 Monoclonal Antibody for Indirect ELISA, IP, WB
Cat No. 68441-1-PBS

产品说明书

CloneNo. 3C11A9

宿主/亚型

Mouse / IgG1

种属反应性

rabbit, pig, rat, mouse, human

应用

Indirect ELISA, IP, WB

别名

V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68441-1-PBS targets ATP6V1B2 in Indirect ELISA, IP, WB applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

经测试应用 Indirect ELISA, IP, WB Application Description
经测试反应性 rabbit, pig, rat, mouse, human
免疫原 ATP6V1B2 fusion protein Ag7265 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
别名 V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2
计算分子量 57 kDa
观测分子量 56-58 kDa
GenBank蛋白编号BC003100
基因名称 ATP6V1B2
Gene ID (NCBI) 526
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP21281
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).