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验证数据展示

  • FC experiment of A431 using CL488-24822

    FC experiment of A431 using CL488-24822

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Atxn2l Polyclonal Antibody (CL488-24822)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL488-24822

    IF Staining of A431 using CL488-24822

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 ATXN2L antibody (CL488-24822) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ATXN2L Polyclonal antibody

ATXN2L Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-24822

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Ataxin-2 domain protein, ataxin 2 like, A2RP, A2LP, A2LG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 24822-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24822 targets ATXN2L in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen ATXN2L fusion protein Ag20581 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ataxin 2-like
Synonyms Ataxin-2 domain protein, ataxin 2 like, A2RP, A2LP, A2LG
Calculated Molecular Weight 1062 aa, 112 kDa
Observed Molecular Weight ~140 kDa
GenBank Accession NumberBC082760
Gene Symbol ATXN2L
Gene ID (NCBI) 11273
RRIDAB_3672775
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWM7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ATXN2L antibody CL488-24822Download protocol
FC protocol for CL Plus 488 ATXN2L antibody CL488-24822Download protocol
Standard Protocols
Click here to view our Standard Protocols