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验证数据展示

  • FC experiment of A431 using CL488-24822

    FC experiment of A431 using CL488-24822

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Atxn2l Polyclonal Antibody (CL488-24822)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL488-24822

    IF Staining of A431 using CL488-24822

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 ATXN2L antibody (CL488-24822) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ATXN2L Polyclonal antibody

ATXN2L Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-24822

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

Ataxin-2 domain protein, ataxin 2 like, A2RP, A2LP, A2LG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 24822-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24822 targets ATXN2L in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 ATXN2L fusion protein Ag20581 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ataxin 2-like
别名 Ataxin-2 domain protein, ataxin 2 like, A2RP, A2LP, A2LG
计算分子量 1062 aa, 112 kDa
观测分子量 ~140 kDa
GenBank蛋白编号BC082760
基因名称 ATXN2L
Gene ID (NCBI) 11273
RRIDAB_3672775
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWM7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ATXN2L antibody CL488-24822Download protocol
FC protocol for CL Plus 488 ATXN2L antibody CL488-24822Download protocol
Standard Protocols
Click here to view our Standard Protocols