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Acetyl-Histone H3 (Lys27) Recombinant antibody, PBS Only

Acetyl-Histone H3 (Lys27) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1M16

Cat no : 82902-1-PBS

Print datasheet

Synonyms

HIST1H3A, 1M16, Histone-H3, HistoneH3, Histone H3

验证数据展示

  • Dot Blot experiment of peptide using 82902-1-RR (same clone as 82902-1-PBS)

    Dot Blot experiment of peptide using 82902-1-RR (same clone as 82902-1-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys27) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (ug/mL) spotted is indicated next to each row. Column 1: H3K27ac. Column 2: Unmodified H3K27. Column 3: H3K9ac. Column 4: H3K14ac. Column 5: H3K18ac. Column 6: H3K23ac. Column 7: H3K36ac. Column 8: H4K5ac. Column 9: H4K8ac. Column 10: H4K12ac. Column 11: H4K16ac. Column 12: H2AK5ac. Column 13: Blank(PBS). This data was developed using the same antibody clone with 82902-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82902-1-RR (same clone as 82902-1-PBS)

    WB analysis of HeLa using 82902-1-RR (same clone as 82902-1-PBS)

    Various cell lysates were subjected to SDS PAGE followed by western blot with 82902-1-RR Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82902-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82902-1-RR (same clone as 82902-1-PBS)

    IHC staining of mouse testis using 82902-1-RR (same clone as 82902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82902-1-RR (Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82902-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IF Staining of HeLa using 82902-1-RR (same clone as 82902-1-PBS)

    IF Staining of HeLa using 82902-1-RR (same clone as 82902-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys27) antibody (82902-1-RR, Clone: 1M16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82902-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

产品信息

82902-1-PBS targets Acetyl-Histone H3 (Lys27) in WB, IHC, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Dot Blot, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 1M16, Histone-H3, HistoneH3, Histone H3
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes. Acetyl-Histone H3 (Lys27) is enhancer specific mark and plays positive role in gene expression.