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Acetyl-Histone H3 (Lys9) Recombinant antibody, PBS Only

Acetyl-Histone H3 (Lys9) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241596F9

Cat no : 84543-1-PBS

Print datasheet

Synonyms

HIST1H3A, 241596F9, Histone-H3, HistoneH3, Histone H3

验证数据展示

  • Dot Blot experiment of peptide using 84543-1-RR (same clone as 84543-1-PBS)

    Dot Blot experiment of peptide using 84543-1-RR (same clone as 84543-1-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 84543-1-RR (same clone as 84543-1-PBS)

    WB analysis of HEK-293 using 84543-1-RR (same clone as 84543-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

    WB analysis of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

    Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (HIST1H3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

    IF Staining of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84543-1-RR, Clone: 241596F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

产品信息

84543-1-PBS targets Acetyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Dot Blot, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 241596F9, Histone-H3, HistoneH3, Histone H3
Observed Molecular Weight17 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C