• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Acetyl-Histone H3 (Lys9) Recombinant antibody

Acetyl-Histone H3 (Lys9) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241596F9

Cat no : 84543-1-RR

Print datasheet

Synonyms

HIST1H3A, 241596F9, Histone-H3, HistoneH3, Histone H3

验证数据展示

  • Dot Blot experiment of peptide using 84543-1-RR

    Dot Blot experiment of peptide using 84543-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis of HEK-293 using 84543-1-RR

    WB analysis of HEK-293 using 84543-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 84543-1-RR

    WB analysis of HeLa using 84543-1-RR

    Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (HIST1H3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84543-1-RR

    IF Staining of HeLa using 84543-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84543-1-RR, Clone: 241596F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inTrichostatin A treated HeLa cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84543-1-RR targets Acetyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Dot Blot, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 241596F9, Histone-H3, HistoneH3, Histone H3
Observed Molecular Weight17 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys9) antibody 84543-1-RRDownload protocol
IF protocol for Acetyl-Histone H3 (Lys9) antibody 84543-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols