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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AlaRS Polyclonal antibody

AlaRS Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-17394

Print datasheet

Synonyms

AARS, Renal carcinoma antigen NY-REN-42, EC:6.1.1.7, Alanyl-tRNA synthetase, Alanine--tRNA ligase, cytoplasmic

验证数据展示

  • IF Staining of HepG2 using CL488-17394

    IF Staining of HepG2 using CL488-17394

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 AlaRS antibody (CL488-17394) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-17394

    FC experiment of HepG2 using CL488-17394

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Alars Polyclonal Antibody (CL488-17394)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 17394-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17394 targets AlaRS in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen AlaRS fusion protein Ag11294 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alanyl-tRNA synthetase
Synonyms AARS, Renal carcinoma antigen NY-REN-42, EC:6.1.1.7, Alanyl-tRNA synthetase, Alanine--tRNA ligase, cytoplasmic
Calculated Molecular Weight 968 aa, 107 kDa
Observed Molecular Weight 107 kDa
GenBank Accession NumberBC011451
Gene Symbol AlaRS
Gene ID (NCBI) 16
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49588
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AlaRS antibody CL488-17394Download protocol
FC protocol for CL Plus 488 AlaRS antibody CL488-17394Download protocol
Standard Protocols
Click here to view our Standard Protocols