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验证数据展示

  • IF Staining of HeLa using CL594-67715

    IF Staining of HeLa using CL594-67715

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Alix antibody (CL594-67715, Clone: 1H9D9 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Alix Monoclonal antibody

Alix Monoclonal Antibody for IF/ICC
Cat No. CL594-67715

Print datasheet

CloneNo. 1H9D9

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALIX Ready-To-Use IHC Kit. ALIX primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67715-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67715 targets Alix in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Alix fusion protein Ag30437 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name programmed cell death 6 interacting protein
Synonyms AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP
Calculated Molecular Weight 868 aa, 96 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC020066
Gene Symbol Alix
Gene ID (NCBI) 10015
RRIDAB_2934776
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WUM4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

实验方案

Product Specific Protocols
IF protocol for CL594 Alix antibody CL594-67715Download protocol
Standard Protocols
Click here to view our Standard Protocols