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验证数据展示

  • IF Staining of HeLa using CL594-67715

    IF Staining of HeLa using CL594-67715

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Alix antibody (CL594-67715, Clone: 1H9D9 ) at dilution of 1:200.

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  • KD/KO验证

CoraLite®594-conjugated Alix Monoclonal antibody

Alix Monoclonal Antibody for IF/ICC
Cat No. CL594-67715

产品说明书

CloneNo. 1H9D9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

IF/ICC

别名

AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALIX Ready-To-Use IHC Kit. ALIX primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67715-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67715 targets Alix in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat
免疫原 Alix fusion protein Ag30437 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 6 interacting protein
别名 AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP
计算分子量 868 aa, 96 kDa
观测分子量100 kDa
GenBank蛋白编号BC020066
基因名称 Alix
Gene ID (NCBI) 10015
RRIDAB_2934776
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WUM4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

实验方案

Product Specific Protocols
IF protocol for CL594 Alix antibody CL594-67715Download protocol
Standard Protocols
Click here to view our Standard Protocols