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Alpha-2-Macroglobulin Recombinant antibody, PBS Only

Alpha-2-Macroglobulin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241649D2

Cat no : 84347-5-PBS

Print datasheet

Synonyms

A2M, 241649D2, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin

验证数据展示

  • WB analysis of human plasma using 84347-5-RR (same clone as 84347-5-PBS)

    WB analysis of human plasma using 84347-5-RR (same clone as 84347-5-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84347-5-RR (Alpha-2-Macroglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84347-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    FC experiment of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR, Clone:241649D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84347-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    IF Staining of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (84347-5-RR, Clone: 241649D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84347-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84347-5-RR

    Affinity and Kinetic Characterization of 84347-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84347-5-RR against Human Alpha-2-Macroglobulin were performed. The affinity constant is 0.193 nM.

Planning an IHC experiment? We recommend our IHCeasy A2M Ready-To-Use IHC Kit. A2M primary antibody included.

产品信息

The immunogen of 84347-5-PBS is Alpha-2-Macroglobulin Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alpha-2-macroglobulin
Synonyms A2M, 241649D2, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin
Calculated Molecular Weight163kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberNM_000014.6
Gene Symbol Alpha 2 Macroglobulin
Gene ID (NCBI) 2
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01023
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha-2-Macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.