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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human plasma using 84347-5-RR (same clone as 84347-5-PBS)

    WB analysis of human plasma using 84347-5-RR (same clone as 84347-5-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84347-5-RR (Alpha-2-Macroglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84347-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    FC experiment of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR, Clone:241649D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84347-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    IF Staining of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (84347-5-RR, Clone: 241649D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84347-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84347-5-RR

    Affinity and Kinetic Characterization of 84347-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84347-5-RR against Human Alpha-2-Macroglobulin were performed. The affinity constant is 0.193 nM.

Alpha-2-Macroglobulin Recombinant antibody, PBS Only

Alpha-2-Macroglobulin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84347-5-PBS

产品说明书

CloneNo. 241649D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

A2M, 241649D2, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy A2M Ready-To-Use IHC Kit. A2M primary antibody included.

产品信息

84347-5-PBS targets Alpha-2-Macroglobulin in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alpha-2-macroglobulin
别名 A2M, 241649D2, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin
计算分子量163kDa
观测分子量 185 kDa
GenBank蛋白编号NM_000014.6
基因名称 Alpha 2 Macroglobulin
Gene ID (NCBI) 2
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01023
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha-2-Macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.