• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Alpha-2-Macroglobulin Recombinant antibody

Alpha-2-Macroglobulin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241649D2

Cat no : 84347-5-RR

Print datasheet

Synonyms

A2M, 241649D2, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin

验证数据展示

  • WB analysis of human plasma using 84347-5-RR

    WB analysis of human plasma using 84347-5-RR

    human plasma were subjected to SDS PAGE followed by western blot with 84347-5-RR (Alpha-2-Macroglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84347-5-RR

    FC experiment of HepG2 using 84347-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR, Clone:241649D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 84347-5-RR

    IF Staining of HepG2 using 84347-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (84347-5-RR, Clone: 241649D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84347-5-RR

    Affinity and Kinetic Characterization of 84347-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84347-5-RR against Human Alpha-2-Macroglobulin were performed. The affinity constant is 0.193 nM.

经过测试的应用

Positive WB detected inhuman plasma
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy A2M Ready-To-Use IHC Kit. A2M primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84347-5-RR is Alpha-2-Macroglobulin Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alpha-2-macroglobulin
Synonyms A2M, 241649D2, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin
Calculated Molecular Weight163kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberNM_000014.6
Gene Symbol Alpha 2 Macroglobulin
Gene ID (NCBI) 2
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-2-Macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

实验方案

Product Specific Protocols
WB protocol for Alpha-2-Macroglobulin antibody 84347-5-RRDownload protocol
IF protocol for Alpha-2-Macroglobulin antibody 84347-5-RRDownload protocol
FC protocol for Alpha-2-Macroglobulin antibody 84347-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols