验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, rat eye tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IP | See 1 publications below |
产品信息
68001-1-Ig targets Alpha B Crystallin in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | IP |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Alpha B Crystallin fusion protein Ag8656 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | crystallin, alpha B |
| Synonyms | Alpha crystallin B chain, Alpha(B) crystallin, CRYA2, CRYAB, crystallin, alpha B, CTPP2, Heat shock protein beta 5, HSPB5, Rosenthal fiber component |
| Calculated Molecular Weight | 175 aa, 20 kDa |
| Observed Molecular Weight | 20-22 kDa |
| GenBank Accession Number | BC007008 |
| Gene Symbol | Alpha B Crystallin |
| Gene ID (NCBI) | 1410 |
| RRID | AB_2918749 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02511 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha B Crystallin antibody 68001-1-Ig | Download protocol |
| IHC protocol for Alpha B Crystallin antibody 68001-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
