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Alpha B Crystallin Monoclonal antibody, PBS Only

Alpha B Crystallin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4C3

Cat no : 68001-1-PBS

Print datasheet

Synonyms

Alpha crystallin B chain, Alpha(B) crystallin, CRYA2, CRYAB, crystallin, alpha B, CTPP2, Heat shock protein beta 5, HSPB5, Rosenthal fiber component

验证数据展示

  • WB analysis of rat eye using 68001-1-Ig (same clone as 68001-1-PBS)

    WB analysis of rat eye using 68001-1-Ig (same clone as 68001-1-PBS)

    rat eye tissue were subjected to SDS PAGE followed by western blot with 68001-1-Ig (aB-Crystallin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68001-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse eye using 68001-1-Ig (same clone as 68001-1-PBS)

    WB analysis of mouse eye using 68001-1-Ig (same clone as 68001-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 68001-1-Ig (aB-Crystallin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68001-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 68001-1-Ig (same clone as 68001-1-PBS)

    IHC staining of mouse eye using 68001-1-Ig (same clone as 68001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68001-1-Ig (Alpha B Crystallin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68001-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CRYAB antibody included
Planning an IHC experiment? We recommend our IHCeasy CRYAB Ready-To-Use IHC Kit. CRYAB primary antibody included.

产品信息

68001-1-PBS targets Alpha B Crystallin in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Alpha B Crystallin fusion protein Ag8656 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name crystallin, alpha B
Synonyms Alpha crystallin B chain, Alpha(B) crystallin, CRYA2, CRYAB, crystallin, alpha B, CTPP2, Heat shock protein beta 5, HSPB5, Rosenthal fiber component
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight20-22 kDa
GenBank Accession NumberBC007008
Gene Symbol Alpha B Crystallin
Gene ID (NCBI) 1410
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02511
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.