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验证数据展示

  • FC experiment of SH-SY5Y using CL488-14362

    FC experiment of SH-SY5Y using CL488-14362

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Alpha N-Catenin Polyclonal Antibody (CL488-14362)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of SH-SY5Y using CL488-14362

    IF Staining of SH-SY5Y using CL488-14362

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 Alpha N-Catenin antibody (CL488-14362) at dilution of 1:200.

CoraLite® Plus 488-conjugated Alpha N-Catenin Polyclonal antibody

Alpha N-Catenin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-14362

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

CTNNA2, Catenin alpha-2, Catenin alpha 2, CAPR, Alpha-catenin-related protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488

规格: 
100 μL

¥3200/ 100 μL

浓度: 1000 μg/ml

库存：现货

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy CTNNA2 Ready-To-Use IHC Kit. CTNNA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14362-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14362 targets Alpha N-Catenin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Alpha N-Catenin fusion protein Ag5747 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), alpha 2
别名 CTNNA2, Catenin alpha-2, Catenin alpha 2, CAPR, Alpha-catenin-related protein
计算分子量 105 kDa
观测分子量 102 kDa
GenBank蛋白编号BC052996
基因名称 Alpha N-Catenin
Gene ID (NCBI) 1496
RRIDAB_3672587
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP26232
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Alpha N-Catenin antibody CL488-14362Download protocol
FC protocol for CL Plus 488 Alpha N-Catenin antibody CL488-14362Download protocol
Standard Protocols
Click here to view our Standard Protocols