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验证数据展示

  • FC experiment of HepG2 using CL555-14427

    FC experiment of HepG2 using CL555-14427

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human Apolipoprotein AI (CL555-14427) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody(CL555-30000)(Blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 555-conjugated Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for FC (Intra)
Cat No. CL555-14427

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOA1 Ready-To-Use IHC Kit. APOA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14427-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-14427 targets Apolipoprotein AI in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Apolipoprotein AI fusion protein Ag5793 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein A-I
别名 Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1
计算分子量 31 kDa
观测分子量 26-30 kDa
GenBank蛋白编号BC005380
基因名称 APOA1
Gene ID (NCBI) 335
RRIDAB_2934636
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02647
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 Apolipoprotein AI antibody CL555-14427Download protocol
Standard Protocols
Click here to view our Standard Protocols