验证数据展示
经过测试的应用
Positive IF-P detected in | mouse liver tissue |
Positive FC (Intra) detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-14427 targets Apolipoprotein AI in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
经测试应用 | IF-P, FC (Intra) Application Description |
经测试反应性 | human, mouse |
免疫原 | Apolipoprotein AI fusion protein Ag5793 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | apolipoprotein A-I |
别名 | Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1 |
计算分子量 | 31 kDa |
观测分子量 | 26-30 kDa |
GenBank蛋白编号 | BC005380 |
基因名称 | APOA1 |
Gene ID (NCBI) | 335 |
RRID | AB_2919806 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P02647 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 Apolipoprotein AI antibody CL594-14427 | Download protocol |
FC protocol for CL594 Apolipoprotein AI antibody CL594-14427 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |