• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated Apolipoprotein H Polyclonal antibody

Apolipoprotein H Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11892

Print datasheet

Synonyms

Anticardiolipin cofactor, APC inhibitor, Apo H, APOH, Apolipoprotein H, B2G1, B2GPI, Beta 2 glycoprotein 1, Beta 2 glycoprotein I, BG

验证数据展示

  • IF Staining of HepG2 using CL488-11892

    IF Staining of HepG2 using CL488-11892

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Apolipoprotein H antibody (CL488-11892) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-11892

    FC experiment of HepG2 using CL488-11892

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Apolipoprotein H Polyclonal Antibody (CL488-11892)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOH Ready-To-Use IHC Kit. APOH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11892-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11892 targets Apolipoprotein H in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Apolipoprotein H fusion protein Ag2500 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein H (beta-2-glycoprotein I)
Synonyms Anticardiolipin cofactor, APC inhibitor, Apo H, APOH, Apolipoprotein H, B2G1, B2GPI, Beta 2 glycoprotein 1, Beta 2 glycoprotein I, BG
Calculated Molecular Weight 345 aa, 38 kDa
Observed Molecular Weight 43-55 kDa
GenBank Accession NumberBC026283
Gene Symbol APOH
Gene ID (NCBI) 350
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02749
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins. ApoH is a 54 kDa plasma glycoprotein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Apolipoprotein H antibody CL488-11892Download protocol
FC protocol for CL Plus 488 Apolipoprotein H antibody CL488-11892Download protocol
Standard Protocols
Click here to view our Standard Protocols