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  • Featured Product
  • KD/KO Validated

B23/NPM1 Recombinant antibody

B23/NPM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1G4

Cat no : 82030-1-RR

Print datasheet

Synonyms

B23, NPM1, Numatrin, Nucleophosmin, NPM

验证数据展示

  • WB analysis using 82030-1-RR

    WB analysis using 82030-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82030-1-RR (B23 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 82030-1-RR

    WB analysis of HepG2 using 82030-1-RR

    WB result of B23/NPM1 antibody (82030-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B23/NPM1 transfected HepG2 cells.

  • IHC staining of human prostate cancer using 82030-1-RR

    IHC staining of human prostate cancer using 82030-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NPM1 antibody included
  • IHC staining of human prostate cancer using 82030-1-RR

    IHC staining of human prostate cancer using 82030-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NPM1 antibody included
  • IF Staining of HeLa using 82030-1-RR

    IF Staining of HeLa using 82030-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using B23 antibody (82030-1-RR, Clone: 1G4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of Jurkat using 82030-1-RR

    IP experiment of Jurkat using 82030-1-RR

    IP result of anti-B23/NPM1 (IP:82030-1-RR, 4ug; Detection:82030-1-RR 1:8000) with Jurkat cells lysate 1560 ug.

  • IHC staining of human normal colon using 82030-1-RR

    IHC staining of human normal colon using 82030-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NPM1 antibody included
  • Affinity and Kinetic Characterization of 82030-1-RR

    Affinity and Kinetic Characterization of 82030-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82030-1-RR against Human B23/NPM1 were performed. The affinity constant is 0.409 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, THP-1 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman prostate cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NPM1 Ready-To-Use IHC Kit. NPM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82030-1-RR targets B23/NPM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen B23/NPM1 fusion protein Ag0286 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Synonyms B23, NPM1, Numatrin, Nucleophosmin, NPM
Calculated Molecular Weight 33 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC002398
Gene Symbol NPM1
Gene ID (NCBI) 4869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

实验方案

Product Specific Protocols
WB protocol for B23/NPM1 antibody 82030-1-RRDownload protocol
IHC protocol for B23/NPM1 antibody 82030-1-RRDownload protocol
IF protocol for B23/NPM1 antibody 82030-1-RRDownload protocol
IP protocol for B23/NPM1 antibody 82030-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols