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B23/NPM1 Recombinant antibody, PBS Only

B23/NPM1 Recombinant Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4

Cat No : 82030-1-PBS

Print datasheet

Synonyms

B23, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

验证数据展示

  • WB analysis using 82030-1-RR (same clone as 82030-1-PBS)

    WB analysis using 82030-1-RR (same clone as 82030-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82030-1-RR (B23 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82030-1-RR (same clone as 82030-1-PBS)

    IHC staining of human prostate cancer using 82030-1-RR (same clone as 82030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NPM1 antibody included
  • IHC staining of human prostate cancer using 82030-1-RR (same clone as 82030-1-PBS)

    IHC staining of human prostate cancer using 82030-1-RR (same clone as 82030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NPM1 antibody included
  • IF Staining of HeLa using 82030-1-RR (same clone as 82030-1-PBS)

    IF Staining of HeLa using 82030-1-RR (same clone as 82030-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using B23 antibody (82030-1-RR, Clone: 1G4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 82030-1-RR (same clone as 82030-1-PBS)

    IP experiment of Jurkat using 82030-1-RR (same clone as 82030-1-PBS)

    IP result of anti-B23/NPM1 (IP:82030-1-RR, 4ug; Detection:82030-1-RR 1:8000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • Affinity experiments of 82030-1-RR

    Affinity experiments of 82030-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82030-1-RR against Human B23/NPM1 were performed. The affinity constant is 0.409 nM.

Planning an IHC experiment? We recommend our IHCeasy NPM1 Ready-To-Use IHC Kit. NPM1 primary antibody included.

产品信息

82030-1-PBS targets B23/NPM1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IP, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen B23/NPM1 fusion protein Ag0286 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Synonyms B23, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin
Calculated Molecular Weight 33 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC002398
Gene Symbol NPM1
Gene ID (NCBI) 4869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06748
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.