IHCeasy NPM1 Ready-To-Use IHC Kit

NPM1 Ready-to-use reagent kit for IHC.

Reactivity

Human, Rat

Sample Type

FFPE tissue

Cat No : KHC0621

Synonyms

B23, B23/NPM1, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin



Product Information

KHC0621 is a ready-to-use IHC kit for staining of NPM1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy NPM1 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Mouse Monoclonal
Secondary antibody type Polymer-HRP-Goat anti-Mouse
Reactivity Human, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms B23, B23/NPM1, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin