验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL568-60096 targets B23/NPM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | B23/NPM1 fusion protein Ag7415 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | nucleophosmin (nucleolar phosphoprotein B23, numatrin) |
| 别名 | B23, NPM1, Numatrin, Nucleophosmin, NPM |
| 计算分子量 | 33 kDa |
| 观测分子量 | 35-38 kDa |
| GenBank蛋白编号 | BC002398 |
| 基因名称 | NPM1 |
| Gene ID (NCBI) | 4869 |
| RRID | AB_2919713 |
| 偶联类型 | CoraLite®568 Fluorescent Dye |
| 最大激发/发射波长 | 568 nm / 587 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P06748 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL568 B23/NPM1 antibody CL568-60096 | Download protocol |
| FC protocol for CL568 B23/NPM1 antibody CL568-60096 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |