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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated B23/NPM1 Monoclonal antibody

B23/NPM1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4F12A3

Cat no : CL488-60096

Print datasheet

Synonyms

B23, NPM1, Numatrin, Nucleophosmin, NPM

验证数据展示

  • IF Staining of HepG2 using CL488-60096

    IF Staining of HepG2 using CL488-60096

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-60096 (B23/NPM1 antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL488-60096

    FC experiment of HeLa using CL488-60096

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human B23/NPM1 (CL488-60096, Clone:4F12A3) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NPM1 Ready-To-Use IHC Kit. NPM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60096-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60096 targets B23/NPM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen B23/NPM1 fusion protein Ag7415 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Synonyms B23, NPM1, Numatrin, Nucleophosmin, NPM
Calculated Molecular Weight 33 kDa
GenBank Accession NumberBC002398
Gene Symbol NPM1
Gene ID (NCBI) 4869
RRIDAB_2883101
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06748
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 B23/NPM1 antibody CL488-60096Download protocol
FC protocol for CL Plus 488 B23/NPM1 antibody CL488-60096Download protocol
Standard Protocols
Click here to view our Standard Protocols