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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BAG6 Monoclonal antibody

BAG6 Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B8D3

Cat no : CL488-66661

Print datasheet

Synonyms

BAT3, BCL2-associated athanogene 6, BAG-6, BAG family molecular chaperone regulator 6, BAG 6

验证数据展示

  • WB analysis using CL488-66661

    WB analysis using CL488-66661

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66661 (BAG6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-66661

    FC experiment of HeLa using CL488-66661

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human BAG6 (CL488-66661, Clone:1B8D3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, NIH/3T3 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66661-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66661 targets BAG6 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen BAG6 fusion protein Ag24525 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name HLA-B associated transcript 3
Synonyms BAT3, BCL2-associated athanogene 6, BAG-6, BAG family molecular chaperone regulator 6, BAG 6
Calculated Molecular Weight 119 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC003133
Gene Symbol BAT3/BAG-6
Gene ID (NCBI) 7917
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46379
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 BAG6 antibody CL488-66661Download protocol
FC protocol for CL Plus 488 BAG6 antibody CL488-66661Download protocol
Standard Protocols
Click here to view our Standard Protocols