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CoraLite®594-conjugated BAG6 Monoclonal antibody
BAG6 Monoclonal Antibody for WB, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1B8D3
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, LNCaP cells, HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66661-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66661 targets BAG6 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | BAG6 fusion protein Ag24525 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | HLA-B associated transcript 3 |
Synonyms | BAT3, BCL2-associated athanogene 6, BAG-6, BAG family molecular chaperone regulator 6, BAG 6 |
Calculated Molecular Weight | 119 kDa |
Observed Molecular Weight | 150-160 kDa |
GenBank Accession Number | BC003133 |
Gene Symbol | BAT3/BAG-6 |
Gene ID (NCBI) | 7917 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P46379 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.
实验方案
Product Specific Protocols | |
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WB protocol for CL594 BAG6 antibody CL594-66661 | Download protocol |
FC protocol for CL594 BAG6 antibody CL594-66661 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |