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验证数据展示

  • WB analysis using CL594-66661

    WB analysis using CL594-66661

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66661 (BAG6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL594-66661

    FC experiment of HeLa using CL594-66661

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BAG6 (CL594-66661, Clone:1B8D3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated BAG6 Monoclonal antibody

BAG6 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL594-66661

产品说明书

CloneNo. 1B8D3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

BAT3, BCL2-associated athanogene 6, BAG-6, BAG family molecular chaperone regulator 6, BAG 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66661-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66661 targets BAG6 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 BAG6 fusion protein Ag24525 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 HLA-B associated transcript 3
别名 BAT3, BCL2-associated athanogene 6, BAG-6, BAG family molecular chaperone regulator 6, BAG 6
计算分子量 119 kDa
观测分子量 150-160 kDa
GenBank蛋白编号BC003133
基因名称 BAT3/BAG-6
Gene ID (NCBI) 7917
RRIDAB_3084719
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP46379
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.

实验方案

Product Specific Protocols
WB protocol for CL594 BAG6 antibody CL594-66661Download protocol
FC protocol for CL594 BAG6 antibody CL594-66661Download protocol
Standard Protocols
Click here to view our Standard Protocols