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验证数据展示

  • IF Staining of HeLa using CL488-11200

    IF Staining of HeLa using CL488-11200

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 BAP31 antibody (CL488-11200) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-11200

    FC experiment of HeLa using CL488-11200

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human BAP31 (CL488-11200) (red) or 0.4 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated BAP31 Polyclonal antibody

BAP31 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11200

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

6C6 AG, BAP31, BCAP31, CDM, DXS1357E, p28, Protein CDM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BCAP31 Ready-To-Use IHC Kit. BCAP31 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11200-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11200 targets BAP31 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 BAP31 fusion protein Ag1668 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell receptor-associated protein 31
别名 6C6 AG, BAP31, BCAP31, CDM, DXS1357E, p28, Protein CDM
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC014323
基因名称 BAP31
Gene ID (NCBI) 10134
RRIDAB_2919015
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51572
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BAP31 antibody CL488-11200Download protocol
FC protocol for CL Plus 488 BAP31 antibody CL488-11200Download protocol
Standard Protocols
Click here to view our Standard Protocols