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验证数据展示

  • FC experiment of HeLa using CL647-11200

    FC experiment of HeLa using CL647-11200

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human BAP31 (CL647-11200) (red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated BAP31 Polyclonal antibody

BAP31 Polyclonal Antibody for FC (Intra)
Cat No. CL647-11200

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

6C6 AG, BAP31, BCAP31, CDM, DXS1357E, p28, Protein CDM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BCAP31 Ready-To-Use IHC Kit. BCAP31 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11200-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-11200 targets BAP31 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 BAP31 fusion protein Ag1668 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell receptor-associated protein 31
别名 6C6 AG, BAP31, BCAP31, CDM, DXS1357E, p28, Protein CDM
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC014323
基因名称 BAP31
Gene ID (NCBI) 10134
RRIDAB_2934849
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51572
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 BAP31 antibody CL647-11200Download protocol
Standard Protocols
Click here to view our Standard Protocols