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BARHL2 Recombinant antibody, PBS Only (Detector)

BARHL2 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240558G8

Cat No : 83596-4-PBS

Print datasheet

Synonyms

BarH-like 2 homeobox protein, BarH like 2 homeobox protein, 240558G8

验证数据展示

  • WB analysis using 83596-4-RR (same clone as 83596-4-PBS)

    WB analysis using 83596-4-RR (same clone as 83596-4-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83596-4-RR (BARHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00582-2

    Cytometric bead array standard curve of MP00582-2

    Cytometric bead array standard curve of MP00582-2, BARHL2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83596-1-PBS. Detection antibody: 83596-4-PBS. Standard: Ag21156. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83596-4-RR

    Affinity and Kinetic Characterization of 83596-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83596-4-RR against Human BARHL2 were performed. The affinity constant is 0.478 nM.

产品信息

83596-4-PBS targets BARHL2 as part of a matched antibody pair:

MP00582-2: 83596-1-PBS capture and 83596-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BARHL2 fusion protein Ag21156 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name BarH-like homeobox 2
Synonyms BarH-like 2 homeobox protein, BarH like 2 homeobox protein, 240558G8
Calculated Molecular Weight 387 aa, 42 kDa
GenBank Accession NumberBC126439
Gene Symbol BARHL2
Gene ID (NCBI) 343472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NY43
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C