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验证数据展示

  • IF Staining of U2OS using CL488-82910-2

    IF Staining of U2OS using CL488-82910-2

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 BAZ2A antibody (CL488-82910-2, Clone: 230205B8 ) at dilution of 1:200.

  • FC experiment of A549 using CL488-82910-2

    FC experiment of A549 using CL488-82910-2

    1x10^6 A549 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated BAZ2A Recombinant antibody (CL488-82910-2, Clone:230205B8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated BAZ2A Recombinant antibody

BAZ2A Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82910-2

产品说明书

CloneNo. 230205B8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 82910-2-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82910-2 targets BAZ2A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 BAZ2A fusion protein Ag33963 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bromodomain adjacent to zinc finger domain, 2A
别名 WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3
计算分子量211 kDa
观测分子量250-270 kDa
GenBank蛋白编号NM_013449.4
基因名称 BAZ2A
Gene ID (NCBI) 11176
RRIDAB_3673111
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UIF9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BAZ2A antibody CL488-82910-2Download protocol
FC protocol for CL Plus 488 BAZ2A antibody CL488-82910-2Download protocol
Standard Protocols
Click here to view our Standard Protocols