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验证数据展示

  • WB analysis using 84584-5-RR

    WB analysis using 84584-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84584-5-RR (BAP31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84584-5-RR

    IHC staining of human colon cancer using 84584-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCAP31 antibody included
  • IHC staining of human colon cancer using 84584-5-RR

    IHC staining of human colon cancer using 84584-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCAP31 antibody included
  • FC experiment of HepG2 using 84584-5-RR

    FC experiment of HepG2 using 84584-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug BAP31 Recombinant antibody (84584-5-RR, Clone:241978D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 84584-5-RR

    IF Staining of HeLa using 84584-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (84584-5-RR, Clone: 241978D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84584-5-RR

    Affinity and Kinetic Characterization of 84584-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84584-5-RR against Human BAP31 were performed. The affinity constant is below 1 pM.

BAP31 Recombinant antibody

BAP31 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84584-5-RR

产品说明书

CloneNo. 241978D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

BCAP31, BCR-associated protein 31, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, 6C6 AG

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, MCF-7 cells, THP-1 cells, mouse liver tissue, rat liver tisssue
Positive IHC detected inhuman colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy BCAP31 Ready-To-Use IHC Kit. BCAP31 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84584-5-RR targets BAP31 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BAP31 fusion protein Ag1668 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell receptor-associated protein 31
别名 BCAP31, BCR-associated protein 31, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, 6C6 AG
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC014323
基因名称 BAP31
Gene ID (NCBI) 10134
RRIDAB_3672082
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP51572
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAP31, also known as BCAP31, is a chaperone protein abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded proteins, and their targeting to the ER-associated-degradation (ERAD). CDIP1 and BAP31 interact upon ER stress to regulate the mitochondrial apoptosis pathway(PMID: 24139803). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

实验方案

Product Specific Protocols
WB protocol for BAP31 antibody 84584-5-RRDownload protocol
IHC protocol for BAP31 antibody 84584-5-RRDownload protocol
IF protocol for BAP31 antibody 84584-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols