验证数据展示
经过测试的应用
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
Biotin-66340 targets BCL6 in IHC applications and shows reactivity with human samples.
经测试应用 | IHC Application Description |
经测试反应性 | human |
免疫原 | BCL6 fusion protein Ag15519 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | B-cell CLL/lymphoma 6 |
别名 | B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51 |
计算分子量 | 706 aa, 79 kDa |
GenBank蛋白编号 | BC150184 |
基因名称 | BCL6 |
Gene ID (NCBI) | 604 |
RRID | AB_2883070 |
偶联类型 | Biotin |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P41182 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages. This antibody only recognize human species.
实验方案
Product Specific Protocols | |
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IHC protocol for Biotin BCL6 antibody Biotin-66340 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |