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CoraLite® Plus 647-conjugated BCL6 Monoclonal antibody

BCL6 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E6B1

Cat no : CL647-66340

Print datasheet

Synonyms

B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51

验证数据展示

  • FC experiment of Ramos using CL647-66340

    FC experiment of Ramos using CL647-66340

    1x10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated BCL6 Monoclonal antibody (CL647-66340, Clone:1E6B1)(red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inRamos cells
Positive FC detected inRamos cells
For other applications, we recommend the unconjugated version of this antibody, 66340-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66340 targets BCL6 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen BCL6 fusion protein Ag15519 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name B-cell CLL/lymphoma 6
Synonyms B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51
Calculated Molecular Weight 706 aa, 79 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC150184
Gene Symbol BCL6
Gene ID (NCBI) 604
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP41182
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages. This antibody only recognize human species.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 BCL6 antibody CL647-66340Download protocol
Standard Protocols
Click here to view our Standard Protocols