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验证数据展示

  • FC experiment of K-562 using CL488-67260

    FC experiment of K-562 using CL488-67260

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human BCR (CL488-67260, Clone:2C2E3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated BCR Monoclonal antibody

BCR Monoclonal Antibody for FC (Intra)
Cat No. CL488-67260

产品说明书

CloneNo. 2C2E3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat

应用

FC (Intra)

别名

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy BCR Ready-To-Use IHC Kit. BCR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67260-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67260 targets BCR in FC (Intra) applications and shows reactivity with Human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rat
免疫原 BCR fusion protein Ag28953 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 breakpoint cluster region
别名 ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL
计算分子量 143 kDa
观测分子量 150-160 kDa
GenBank蛋白编号BC066122
基因名称 BCR
Gene ID (NCBI) 613
RRIDAB_3084332
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11274
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 BCR antibody CL488-67260Download protocol
Standard Protocols
Click here to view our Standard Protocols