CoraLite® Plus 488-conjugated BCR Monoclonal antibody
BCR Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2C2E3
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | K-562 cells |
Positive FC detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy BCR Ready-To-Use IHC Kit. BCR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67260-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67260 targets BCR in FC (Intra) applications and shows reactivity with Human, rat samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | Human, rat |
Immunogen | BCR fusion protein Ag28953 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | breakpoint cluster region |
Synonyms | ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL |
Calculated Molecular Weight | 143 kDa |
Observed Molecular Weight | 150-160 kDa |
GenBank Accession Number | BC066122 |
Gene Symbol | BCR |
Gene ID (NCBI) | 613 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P11274 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 BCR antibody CL488-67260 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |