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验证数据展示

  • WB analysis of Jurkat using 67260-1-Ig

    WB analysis of Jurkat using 67260-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 67260-1-Ig

    FC experiment of K-562 using 67260-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human BCR (67260-1-Ig, Clone:2C2E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

BCR Monoclonal antibody

BCR Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 67260-1-Ig
CloneNo. 2C2E3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, ELISA, FC (Intra)

Synonyms

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive FC (Intra) detected inK-562 cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy BCR Ready-To-Use IHC Kit. BCR primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67260-1-Ig targets BCR in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Applications WB, ELISA, FC (Intra) Application Description
Tested Reactivity Human, rat
Immunogen BCR fusion protein Ag28953 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name breakpoint cluster region
Synonyms ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC066122
Gene Symbol BCR
Gene ID (NCBI) 613
RRIDAB_2882531
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11274
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

实验方案

Product Specific Protocols
WB protocol for BCR antibody 67260-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols