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验证数据展示

  • WB analysis of Jurkat using 67260-1-Ig

    WB analysis of Jurkat using 67260-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 67260-1-Ig

    FC experiment of K-562 using 67260-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human BCR (67260-1-Ig, Clone:2C2E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

BCR Monoclonal antibody

BCR Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 67260-1-Ig

产品说明书

CloneNo. 2C2E3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat

应用

WB, ELISA, FC (Intra)

别名

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive FC (Intra) detected inK-562 cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy BCR Ready-To-Use IHC Kit. BCR primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67260-1-Ig targets BCR in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
经测试反应性 Human, rat
免疫原 BCR fusion protein Ag28953 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 breakpoint cluster region
别名 ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL
计算分子量 143 kDa
观测分子量 150-160 kDa
GenBank蛋白编号BC066122
基因名称 BCR
Gene ID (NCBI) 613
RRIDAB_2882531
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11274
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

实验方案

Product Specific Protocols
WB protocol for BCR antibody 67260-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols