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BCR Monoclonal antibody, PBS Only

BCR Monoclonal Antibody for WB,Indirect ELISA,FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB,Indirect ELISA,FC

Conjugate

Unconjugated

CloneNo.

2C2E3

Cat No : 67260-1-PBS

Print datasheet

Synonyms

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

验证数据展示

  • WB analysis of Jurkat using 67260-1-Ig (same clone as 67260-1-PBS)

    WB analysis of Jurkat using 67260-1-Ig (same clone as 67260-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67260-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 67260-1-Ig (same clone as 67260-1-PBS)

    FC experiment of K-562 using 67260-1-Ig (same clone as 67260-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human BCR (67260-1-Ig, Clone:2C2E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67260-1-PBS in a different storage buffer formulation.

产品信息

67260-1-PBS targets BCR in WB, FC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB,Indirect ELISA,FC Application Description
Tested Reactivity Human, rat
Immunogen BCR fusion protein Ag28953 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name breakpoint cluster region
Synonyms ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC066122
Gene Symbol BCR
Gene ID (NCBI) 613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11274
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.