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CoraLite® Plus 488-conjugated BDNF Monoclonal antibody

BDNF Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E5C5

Cat no : CL488-66292

Print datasheet

Synonyms

Abrineurin, BDNF

验证数据展示

  • IF Staining of PC-12 using CL488-66292

    IF Staining of PC-12 using CL488-66292

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CL488-66292 (BDNF antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 66292-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66292 targets BDNF in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen BDNF fusion protein Ag11329 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name brain-derived neurotrophic factor
Synonyms Abrineurin, BDNF
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC029795
Gene Symbol BDNF
Gene ID (NCBI) 627
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23560
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BDNF antibody CL488-66292Download protocol
Standard Protocols
Click here to view our Standard Protocols