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CoraLite®594-conjugated BDNF Monoclonal antibody

BDNF Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E5C5

Cat no : CL594-66292

Print datasheet

Synonyms

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5

验证数据展示

  • IF Staining of PC-12 using CL594-66292

    IF Staining of PC-12 using CL594-66292

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CL594-66292 (BDNF antibody) at dilution of 1:100.

  • FC experiment of U-251 using CL594-66292

    FC experiment of U-251 using CL594-66292

    1x10^6 U-251 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated BDNF Monoclonal antibody (CL594-66292, Clone:1E5C5)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inPC-12 cells
Positive FC (Intra) detected inU-251 cells
For other applications, we recommend the unconjugated version of this antibody, 66292-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66292 targets BDNF in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen BDNF fusion protein Ag11329 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name brain-derived neurotrophic factor
Synonyms Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC029795
Gene Symbol BDNF
Gene ID (NCBI) 627
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23560
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

实验方案

Product Specific Protocols
IF protocol for CL594 BDNF antibody CL594-66292Download protocol
Standard Protocols
Click here to view our Standard Protocols