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验证数据展示

  • WB analysis using 83920-6-RR (same clone as 83920-6-PBS)

    WB analysis using 83920-6-RR (same clone as 83920-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83920-6-RR (BUB1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83920-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 83920-6-RR (same clone as 83920-6-PBS)

    IHC staining of mouse lung using 83920-6-RR (same clone as 83920-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83920-6-RR (BUB1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83920-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BUB1B antibody included
  • IF Staining of HeLa using 83920-6-RR (same clone as 83920-6-PBS)

    IF Staining of HeLa using 83920-6-RR (same clone as 83920-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB1B antibody (83920-6-RR, Clone: 240969F7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83920-6-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83920-6-RR (same clone as 83920-6-PBS)

    WB analysis of HeLa using 83920-6-RR (same clone as 83920-6-PBS)

    WB result of BUB1B antibody (83920-6-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BUB1B transfected HeLa cells. This data was developed using the same antibody clone with 83920-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83920-6-RR

    Affinity and Kinetic Characterization of 83920-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83920-6-RR against Human BUB1B were performed. The affinity constant is 7.60 nM.

  • Featured Product
  • KD/KO验证

BUB1B Recombinant monoclonal antibody, PBS Only

BUB1B Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83920-6-PBS

产品说明书

CloneNo. 240969F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

BubR1, 240969F7, Bub1A, BUB1beta, EC:2.7.11.1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BUB1B Ready-To-Use IHC Kit. BUB1B primary antibody included.

产品信息

83920-6-PBS targets BUB1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34182

Product name: Recombinant human BUB1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 719-1050 aa of BC018739

Sequence: QSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 budding uninhibited by benzimidazoles 1 homolog beta (yeast)
别名 BubR1, 240969F7, Bub1A, BUB1beta, EC:2.7.11.1
计算分子量 1050 aa, 120 kDa
观测分子量130 kDa
GenBank蛋白编号BC018739
基因名称 BubR1
Gene ID (NCBI) 701
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO60566
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3, and SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID: 22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications, including acetylation(PMID: 22167194). Defects in BUB1B are the cause of the premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1). Research has shown that a BUB1B isoform with a measured MW of about 65 kDa (PMID: 34620840).

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