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验证数据展示

  • WB analysis using 83920-6-RR

    WB analysis using 83920-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83920-6-RR (BUB1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 83920-6-RR

    IHC staining of mouse lung using 83920-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83920-6-RR (BUB1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BUB1B antibody included
  • IF Staining of HeLa using 83920-6-RR

    IF Staining of HeLa using 83920-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB1B antibody (83920-6-RR, Clone: 240969F7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 83920-6-RR

    Affinity and Kinetic Characterization of 83920-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83920-6-RR against Human BUB1B were performed. The affinity constant is 7.60 nM.

BUB1B Recombinant antibody

BUB1B Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83920-6-RR

产品说明书

CloneNo. 240969F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Raji cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BUB1B Ready-To-Use IHC Kit. BUB1B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83920-6-RR targets BUB1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 BUB1B fusion protein Ag34182 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 budding uninhibited by benzimidazoles 1 homolog beta (yeast)
别名 BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A
计算分子量 1050 aa, 120 kDa
观测分子量130 kDa
GenBank蛋白编号BC018739
基因名称 BubR1
Gene ID (NCBI) 701
RRIDAB_3671500
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60566
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B cause premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

实验方案

Product Specific Protocols
WB protocol for BUB1B antibody 83920-6-RRDownload protocol
IHC protocol for BUB1B antibody 83920-6-RRDownload protocol
IF protocol for BUB1B antibody 83920-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols