验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83920-6 targets BUB1B in IF/ICC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | BUB1B fusion protein Ag34182 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | budding uninhibited by benzimidazoles 1 homolog beta (yeast) |
| 别名 | |
| 计算分子量 | 1050 aa, 120 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC018739 |
| 基因名称 | BubR1 |
| Gene ID (NCBI) | 701 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O60566 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B cause premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 BUB1B antibody CL488-83920-6 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |