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Beta-2-Microglobulin Recombinant antibody

Beta-2-Microglobulin Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

240660A12

Cat no : 83683-6-RR

Print datasheet

Synonyms

B2M, 240660A12, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

验证数据展示

  • WB analysis using 83683-6-RR

    WB analysis using 83683-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 83683-6-RR

    IP experiment of A431 using 83683-6-RR

    IP result of anti-Beta-2-Microglobulin (IP:83683-6-RR, 4ug; Detection:83683-6-RR 1:3000) with A431 cells lysate 2180 ug.

  • Affinity and Kinetic Characterization of 83683-6-RR

    Affinity and Kinetic Characterization of 83683-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83683-6-RR against Human Beta-2-Microglobulin were performed. The affinity constant is 1.18 nM.

经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, Raji cells, U-937 cells, Jurkat cells
Positive IP detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83683-6-RR is Beta-2-Microglobulin Fusion Protein expressed in E. coli.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen Beta-2-Microglobulin fusion protein Ag4433 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name beta-2-microglobulin
Synonyms B2M, 240660A12, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3
Calculated Molecular Weight 119 aa, 14 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP61769
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

实验方案

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 83683-6-RRDownload protocol
IP protocol for Beta-2-Microglobulin antibody 83683-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols