验证数据展示
经过测试的应用
| Positive IF/ICC detected in | NCCIT cells |
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13511-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-13511 targets Beta-2-Microglobulin in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Beta-2-Microglobulin fusion protein Ag4433 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | beta-2-microglobulin |
| Synonyms | B2M, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin |
| Calculated Molecular Weight | 119 aa, 14 kDa |
| Observed Molecular Weight | 12-14 kDa |
| GenBank Accession Number | BC032589 |
| Gene Symbol | B2M |
| Gene ID (NCBI) | 567 |
| ENSEMBL Gene ID | ENSG00000166710 |
| RRID | AB_2919075 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61769 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Beta-2-Microglobulin antibody CL488-13511 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |